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用活细胞显微镜研究低压等离子体灭菌对
枯草芽孢杆菌
孢子存活的不利影响
JoVE Journal
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JoVE Journal
Biology
Investigating the Detrimental Effects of Low Pressure Plasma Sterilization on the Survival of
Bacillus subtilis
Spores Using Live Cell Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
用活细胞显微镜研究低压等离子体灭菌对
枯草芽孢杆菌
孢子存活的不利影响
DOI:
10.3791/56666-v
•
10:03 min
•
November 30, 2017
•
Felix M. Fuchs
,
Marina Raguse
2,3
,
Marcel Fiebrandt
,
Kazimierz Madela
,
Peter Awakowicz
,
Michael Laue
,
Katharina Stapelmann
,
Ralf Moeller
1
Department of Radiation Biology, Institute of Aerospace Medicine, Space Microbiology Research Group
,
German Aerospace Center (DLR e.V.)
,
2
Institute of Electrical Engineering and Plasma Technology, Faculty of Electrical Engineering and Information Technology
,
Ruhr-University Bochum
,
3
Institute of Electrical Engineering and Plasma Technology, Faculty of Electrical Engineering and Information Technology, Biomedical Applications of Plasma Technology
,
Ruhr-University Bochum
,
4
Advanced Light and Electron Microscopy (ZBS 4)
,
Robert Koch Institute
Chapters
00:05
Title
00:55
Bacillus subtilis
Spore Production and Purification and Sample Preparation of Aerosol-deposited
Bacillus subtilis
Spores
03:03
Low Pressure Plasma Treatment and Recovery and Evaluation of Spore Survival
05:10
Live Cell Microscopy, Tracking of DNA Repair Processes in Germinating Spores, and Scanning Electron Microscopy
07:27
Results:
Bacillus subtilis
Spore Survival After Low Pressure Plasma Sterilization
09:02
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这项协议说明了评估在用低压等离子体处理后恢复
枯草芽孢杆菌
孢子后, 监测活力参数和 DNA 修复过程的相关性所需的重要连续步骤。荧光标记的 DNA 修复蛋白质通过时间分辨共焦显微镜和扫描电子显微镜。
Tags
Low Pressure Plasma
Bacillus Subtilis Spores
Live Cell Microscopy
DNA Repair
Microbial Inactivation
Sterilization
Spore Production
Spore Purification
Spore Titer
Scanning Electron Microscopy
Fluorescence Microscopy
Spore Suspension
Aerosol Spraying
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