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用活细胞显微镜研究低压等离子体灭菌对枯草芽孢杆菌孢子存活的不利影响
JoVE Journal
Biology
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JoVE Journal Biology
Investigating the Detrimental Effects of Low Pressure Plasma Sterilization on the Survival of Bacillus subtilis Spores Using Live Cell Microscopy
DOI:

10:03 min

November 30, 2017

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Chapters

  • 00:05Title
  • 00:55Bacillus subtilis Spore Production and Purification and Sample Preparation of Aerosol-deposited Bacillus subtilis Spores
  • 03:03Low Pressure Plasma Treatment and Recovery and Evaluation of Spore Survival
  • 05:10Live Cell Microscopy, Tracking of DNA Repair Processes in Germinating Spores, and Scanning Electron Microscopy
  • 07:27Results: Bacillus subtilis Spore Survival After Low Pressure Plasma Sterilization
  • 09:02Conclusion

Summary

Automatic Translation

这项协议说明了评估在用低压等离子体处理后恢复枯草芽孢杆菌孢子后, 监测活力参数和 DNA 修复过程的相关性所需的重要连续步骤。荧光标记的 DNA 修复蛋白质通过时间分辨共焦显微镜和扫描电子显微镜。

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