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1Department of Radiation Biology, Institute of Aerospace Medicine, Space Microbiology Research Group,German Aerospace Center (DLR e.V.), 2Institute of Electrical Engineering and Plasma Technology, Faculty of Electrical Engineering and Information Technology,Ruhr-University Bochum, 3Institute of Electrical Engineering and Plasma Technology, Faculty of Electrical Engineering and Information Technology, Biomedical Applications of Plasma Technology,Ruhr-University Bochum, 4Advanced Light and Electron Microscopy (ZBS 4),Robert Koch Institute
Chapters
- 00:05Title
- 00:55Bacillus subtilis Spore Production and Purification and Sample Preparation of Aerosol-deposited Bacillus subtilis Spores
- 03:03Low Pressure Plasma Treatment and Recovery and Evaluation of Spore Survival
- 05:10Live Cell Microscopy, Tracking of DNA Repair Processes in Germinating Spores, and Scanning Electron Microscopy
- 07:27Results: Bacillus subtilis Spore Survival After Low Pressure Plasma Sterilization
- 09:02Conclusion
Este protocolo ilustra as etapas consecutivas importantes, necessárias para avaliar a pertinência de monitoramento parâmetro de vitalidade e os processos de reparação do DNA em reviving esporos de Bacillus subtilis após o tratamento com plasma de baixa pressão de tracking proteínas através do tempo-resolvido microscopia confocal e microscopia eletrônica de reparo de DNA marcado com fluorescência.
