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Undersøge de skadelige effekter af lav pres Plasma sterilisation på overlevelse af Bacillus subtilis sporer ved hjælp af Live celle mikroskopi
JoVE Journal
Biology
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JoVE Journal Biology
Investigating the Detrimental Effects of Low Pressure Plasma Sterilization on the Survival of Bacillus subtilis Spores Using Live Cell Microscopy
DOI:

10:03 min

November 30, 2017

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Chapters

  • 00:05Title
  • 00:55Bacillus subtilis Spore Production and Purification and Sample Preparation of Aerosol-deposited Bacillus subtilis Spores
  • 03:03Low Pressure Plasma Treatment and Recovery and Evaluation of Spore Survival
  • 05:10Live Cell Microscopy, Tracking of DNA Repair Processes in Germinating Spores, and Scanning Electron Microscopy
  • 07:27Results: Bacillus subtilis Spore Survival After Low Pressure Plasma Sterilization
  • 09:02Conclusion

Summary

Automatic Translation

Denne protokol illustrerer de vigtige træk skridt forpligtet til at vurdere relevansen af overvågning vitalitet parameter og DNA reparationsprocesser i genoplive Bacillus subtilis sporer efter behandling med lavt tryk plasma med tracking Fluorescens-mærket DNA reparere proteiner via tidsopløst Fluorescens mikroskopi og scanning Elektron Mikroskopi.

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