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提高吞吐量的轰击大麦糊粉层层中基因表达的测定
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Genetics
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JoVE Journal Genetics
Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
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提高吞吐量的轰击大麦糊粉层层中基因表达的测定

DOI:
10.3791/56728-v

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10:29 min

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March 30, 2018

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, , , ,
1Department of Biology,Colby College

Chapters

  • 00:05Title
  • 00:44Preparation of Barley Grains and Plasmid DNA for Bombardment
  • 02:42Particle Bombardment of Embryoless Barley Grains
  • 04:42Extraction of Soluble Protein from the Bombarded Grains
  • 06:24Measurement of GUS Activity from Grain Extracts
  • 08:15Results: Measurement of Gene Expression in Barley Aleurone Cells after Particle Bombardment
  • 09:50Conclusion

Summary

Automatic Translation

Automatic Translation

提出了一种改进的协议, 用于测量粒子轰击后大麦糊粉层细胞中的瞬时基因表达。采用全自动谷物研磨与96井板酶检测相结合, 为该过程提供了较高的吞吐量。

Tags

Gene ExpressionBarley AleuroneParticle BombardmentReporter ConstructsAbscisic AcidHigh-throughputSeed Coat PeelingVermiculiteUBI LuciferaseGUS ReporterEffector PlasmidMicrocarriersParticle Delivery SystemRupture Disk

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