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Chapters
- 00:05Title
- 00:44Preparation of Barley Grains and Plasmid DNA for Bombardment
- 02:42Particle Bombardment of Embryoless Barley Grains
- 04:42Extraction of Soluble Protein from the Bombarded Grains
- 06:24Measurement of GUS Activity from Grain Extracts
- 08:15Results: Measurement of Gene Expression in Barley Aleurone Cells after Particle Bombardment
- 09:50Conclusion
En forbedret protokol præsenteres for måling af forbigående genekspression fra reporter konstruktioner i Byg aleurone celler efter partikel bombardement. Kombinationen af automatiserede korn slibning med 96 brønde plade enzym-assays giver høj overførselshastighed for proceduren.
