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Måling af genekspression i bombarderede Byg Aleurone lag med øget gennemløb
JoVE Journal
Genetics
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JoVE Journal Genetics
Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
DOI:

10:29 min

March 30, 2018

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Chapters

  • 00:05Title
  • 00:44Preparation of Barley Grains and Plasmid DNA for Bombardment
  • 02:42Particle Bombardment of Embryoless Barley Grains
  • 04:42Extraction of Soluble Protein from the Bombarded Grains
  • 06:24Measurement of GUS Activity from Grain Extracts
  • 08:15Results: Measurement of Gene Expression in Barley Aleurone Cells after Particle Bombardment
  • 09:50Conclusion

Summary

Automatic Translation

En forbedret protokol præsenteres for måling af forbigående genekspression fra reporter konstruktioner i Byg aleurone celler efter partikel bombardement. Kombinationen af automatiserede korn slibning med 96 brønde plade enzym-assays giver høj overførselshastighed for proceduren.

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