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Isolasjon, kultur og differensiering av benmarg Stromal celler og Osteoclast Progenitors fra mus
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice
DOI:

08:07 min

January 06, 2018

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Chapters

  • 00:05Title
  • 00:41Collection Tube Preparation and Bone Harvest
  • 02:52Total Bone Marrow Cell (BMC) and Split BMC Population Plating and Culture
  • 04:45Bone Marrow Stem Cell (BMSC) Differentiation
  • 06:07Results: Representative BMSC Differentiation
  • 07:27Conclusion

Summary

Automatic Translation

I denne artikkelen presenterer vi metoder å isolere og skille benmarg stromal celler og blodkreft stamceller fra musen lange ben. To forskjellige protokoller presenteres gir ulike celle populasjoner egnet for ekspansjon og differensiering inn osteoblasts, adipocytter og osteoklast.

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