JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Developmental Biology
/
人诱导多能干细胞的前脑型脑 Organoids 标准化和可再生性的生成
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Generation of Standardized and Reproducible Forebrain-type Cerebral Organoids from Human Induced Pluripotent Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
人诱导多能干细胞的前脑型脑 Organoids 标准化和可再生性的生成
DOI:
10.3791/56768-v
•
10:25 min
•
January 23, 2018
•
Olivia Krefft*
1
,
Ammar Jabali*
1
,
Vira Iefremova
,
Philipp Koch
2,3
,
Julia Ladewig
1
Institute of Reconstructive Neurobiology
,
University of Bonn
,
2
Central Institute of Mental Health
,
University of Heidelberg/Medical Faculty Mannheim
,
3
Hector Institute for Translational Brain Research (HITBR gGmbH)
Chapters
00:05
Title
00:55
iPSC Aggregate Generation
02:30
Anterior Neuroectoderm Induction
03:51
Neuroectodermal Aggregates embedding within a Matrix Scaffold
05:49
Forebrain-type Organoid Generation, Fixation, and Validation
07:58
Results: Representative Forebrain-type Organoid Validation
09:36
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
脑 organoids 代表了一个新的模型系统来研究早期人脑发育
体外
。本文提供了详细的方法, 以有效地产生同种背前脑型 organoids 的人诱导多能干细胞, 包括关键的表征和验证步骤。
Tags
Human Induced Pluripotent Stem Cells
Forebrain-type Cerebral Organoids
Standardized And Reproducible Organoid Generation
Modeling Neurodevelopment
Cortical Genesis
Aggregation
Anterior Neuroectoderm Induction
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
树突棘从锥体神经元的三维定量从人类诱导多能干细胞衍生
从人多能干细胞使用高度可重复的小分子为基础的分化协议心肌细胞规模化生产
从人黑色素瘤肿瘤浸润淋巴细胞诱导多能干细胞的产生
从人体外周血单个核细胞融合,无诱导多能干细胞的生成方法游离型载体
界定和由人多能干细胞的肝细胞样细胞的可扩展代
分化软骨细胞与外周血源性人诱导多能干细胞
从脐带血源性多能干细胞中提取三维皮肤有机细胞
人诱导多能干细胞体外生成体细胞衍生物的研究
从人类诱导多能干细胞诱导的体外神经肌肉结点
从诱导多能干细胞生成3D全肺类器官,用于模拟肺发育生物学和疾病
Read Article