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Metabolic Mapping: Quantitative Enzyme Cytochemistry and Histochemistry to Determine the Activity of Dehydrogenases in Cells and Tissues
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Metabolic Mapping: Quantitative Enzyme Cytochemistry and Histochemistry to Determine the Activity of Dehydrogenases in Cells and Tissues

Metabolic Mapping: Quantitative Enzyme Cytochemistry and Histochemistry to Determine the Activity of Dehydrogenases in Cells and Tissues

DOI:
10.3791/56843-v

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08:36 min

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May 26, 2018

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, , ,
1Cancer Center Amsterdam, Department of Medical Biology, Academic Medical Center,University of Amsterdam, Amsterdam, The Netherlands

Chapters

  • 00:00Title
  • 00:50Preparing Reaction Buffer
  • 03:50Applying Incubation Medium to Tissue Sections
  • 05:32Washing the Microscopy Slides
  • 06:33Enclose the Tissue Sections on Microscopy Slides
  • 07:13Representative Results
  • 08:14Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol that can be used to microscopically visualize and quantify activity of dehydrogenases in cells and tissues and its kinetics, function and subcellular localization.

Tags

Keywords: Metabolic MappingEnzyme CytochemistryHistochemistryDehydrogenase ActivityCellsPolyvinyl AlcoholPVAPhosphate BufferNitroblue Tetrazolium ChlorideNitroBTEthanolDimethylformamideReaction BufferCytospinsMetabolismCancer CellsTherapeutic Target

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