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Immunology and Infection
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代谢图谱: 定量酶化学和组织化学法测定脱氢酶在细胞和器官中的活性
JoVE Journal
Immunology and Infection
Author Produced
This content is Free Access.
JoVE Journal
Immunology and Infection
Metabolic Mapping: Quantitative Enzyme Cytochemistry and Histochemistry to Determine the Activity of Dehydrogenases in Cells and Tissues
Please note that all translations are automatically generated.
Click here for the English version.
代谢图谱: 定量酶化学和组织化学法测定脱氢酶在细胞和器官中的活性
DOI:
10.3791/56843-v
•
08:36 min
•
May 26, 2018
•
Remco J. Molenaar
,
Mohammed Khurshed
,
Vashendriya V.V. Hira
,
Cornelis J.F. Van Noorden
1
Cancer Center Amsterdam, Department of Medical Biology, Academic Medical Center
,
University of Amsterdam, Amsterdam, The Netherlands
Chapters
00:00
Title
00:50
Preparing Reaction Buffer
03:50
Applying Incubation Medium to Tissue Sections
05:32
Washing the Microscopy Slides
06:33
Enclose the Tissue Sections on Microscopy Slides
07:13
Representative Results
08:14
Conclusion
Summary
Automatic Translation
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在这里, 我们提出了一个协议, 可用于显微镜下可视化和量化的活动脱氢酶在细胞和组织及其动力学, 功能和亚细胞定位。
Tags
Metabolic Mapping
Enzyme Cytochemistry
Histochemistry
Dehydrogenase Activity
Cells
Polyvinyl Alcohol
PVA
Phosphate Buffer
Nitroblue Tetrazolium Chloride
NitroBT
Ethanol
Dimethylformamide
Reaction Buffer
Cytospins
Metabolism
Cancer Cells
Therapeutic Target
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