A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Institute of Neuroscience, State Key Laboratory of Neuroscience, Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences, 2College of Life Sciences,University of Chinese Academy of Sciences, 3School of Life Science and Technology,Shanghai Tech University, 4Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences
Chapters
- 00:04Title
- 00:54sgRNA Surveyor Nuclease Assay
- 02:50Cas9 mRNA Production
- 04:11sgRNA Production
- 05:30Embryo Collection, Microinjection, and In Vitro Culture
- 06:43Results: Superior Integration with Cas9
- 07:46Conclusion
Kümelenmiş düzenli olarak kısa palindromik tekrarlar/ilişkili CRISPR protein 9 (CRISPR/Cas9) interspaced sistem genetik mühendisliği için umut verici bir araçtır ve hedeflenen entegrasyonu transgenes olasılığı kadar açılır. Biz (HMEJ) katılmadan homoloji-aracılı son tarif-verimli DNA Tümleştirme vivo içinde hedef ve gen tedavileri CRISPR/Cas9 kullanarak hedef için strateji tabanlı.
