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Journal
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Developmental Biology
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成人皮肤角质形成细胞的生成与培养
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Generation and Culturing of Primary Human Keratinocytes from Adult Skin
Please note that all translations are automatically generated.
Click here for the English version.
成人皮肤角质形成细胞的生成与培养
DOI:
10.3791/56863-v
•
10:42 min
•
December 22, 2017
•
Claus Johansen
1
Department of Dermatology
,
Aarhus University Hospital
Chapters
00:05
Title
00:52
Isolation of Keratinocytes from Human Skin
05:32
Passaging of Keratinocytes
07:32
Freezing Keratinocytes
08:48
Results: Primary Human Keratinocyte Culture from Adult Skin
10:06
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
人类皮肤作为抵御外部环境的第一条防线。我们提出了一种从成人皮肤中分离原代角质形成细胞的方法。这些孤立的角质形成细胞是有用的许多实验设置, 是一个非常适合的模型研究的分子机制在皮肤生物学
体外
.
Tags
Primary Human Keratinocytes
Adult Skin
Epidermal Cell Biology
Skin Diseases
Trypsin
DPBS
KSFM
RPMI 1640
FBS
Gentamicin
Epidermal Cells
Dermal Compartment
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