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Opdigte optisk kvalitet glasoverflader for at studere makrofag Fusion
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Fabricating Optical-quality Glass Surfaces to Study Macrophage Fusion
DOI:

08:50 min

March 14, 2018

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Chapters

  • 00:05Title
  • 00:47Preparing Acid-cleaned Cover Glass
  • 01:59Preparing of Fusogenic Optical-quality Surfaces
  • 02:52Micropatterning Hydrocarbon-containing Compounds to Confine Fusion to Predetermined Regions
  • 03:58Fabricating Glass Bottom Dishes
  • 04:55Collecting Thioglycollate-elicited Macrophages
  • 06:15Results: Macrophage Fusion on Glass Surfaces
  • 07:51Conclusion

Summary

Automatic Translation

Denne protokol beskriver fabrikation af optiske kvalitet glasoverflader adsorberet med forbindelser, der indeholder langkædede carbonhydrider, som kan bruges til at overvåge makrofag fusion af levende enheder og muliggør super-resolution mikroskopi af faste enheder .

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