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Journal
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Immunology and Infection
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マクロファージの融合を研究するための光学的品質ガラス表面を加工
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Fabricating Optical-quality Glass Surfaces to Study Macrophage Fusion
Please note that all translations are automatically generated.
Click here for the English version.
マクロファージの融合を研究するための光学的品質ガラス表面を加工
DOI:
10.3791/56866-v
•
08:50 min
•
March 14, 2018
•
James J. Faust
2
,
Wayne Christenson
4,5
,
Kyle Doudrick
,
John Heddleston
,
Teng-Leong Chew
,
Marko Lampe
,
Arnat Balabiyev
2
,
Robert Ros
4,5
,
Tatiana P. Ugarova
2
1
Center for Metabolic and Vascular Biology
,
Mayo Clinic
,
2
Molecular and Cellular Biosciences, School of Life Sciences
,
Arizona State University
,
3
Department of Physics
,
Arizona State University
,
4
Center for Biological Physics
,
Arizona State University
,
5
Biodesign Institute
,
Arizona State University
,
6
Department of Civil and Environmental Engineering and Earth Sciences
,
University of Notre Dame
,
7
Advanced Imaging Center
,
HHMI Janelia Research Campus
,
8
Advanced Light Microscopy Facility
,
European Molecular Biology Laboratory
Chapters
00:05
Title
00:47
Preparing Acid-cleaned Cover Glass
01:59
Preparing of Fusogenic Optical-quality Surfaces
02:52
Micropatterning Hydrocarbon-containing Compounds to Confine Fusion to Predetermined Regions
03:58
Fabricating Glass Bottom Dishes
04:55
Collecting Thioglycollate-elicited Macrophages
06:15
Results: Macrophage Fusion on Glass Surfaces
07:51
Conclusion
Summary
Automatic Translation
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Automatic Translation
このプロトコルを記述する生きている標本のマクロファージの融合の監視に使用することができ、固定標本の超解像顕微鏡の長鎖炭化水素を含む化合物を吸着した光学的品質のガラス表面の作製.
Tags
Optical Glass
Macrophage Fusion
Microscopy
Acid Cleaning
Paraffin Coating
Gold TEM Grid
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