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Co kultur metode å undersøke Crosstalk mellom X-ray bestrålt Caco-2 celler og PBMC
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
A Co-culture Method to Investigate the Crosstalk Between X-ray Irradiated Caco-2 Cells and PBMC
DOI:

11:40 min

January 30, 2018

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Chapters

  • 00:04Title
  • 01:05Cell Preparation and Irradiation
  • 03:21Cell Metabolic Activity (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide [MTT]) and Viability Assays, and Trans-Epithelial Electrical Resistance (TEER)
  • 06:12Western Blot Analysis
  • 09:15Results: Representative Effects of Caco-2 Cell Radiation Exposure and PBMC Co-culture
  • 11:01Conclusion

Summary

Automatic Translation

Vi presenterer en protokoll for å undersøke crosstalk mellom X-ray-bestrålt Caco-2 og perifert blod mononukleære celler (PBMC). Protokollen starter med Caco-2 bestråling og oppsett av co kultur med PBMC; deretter måles trans-epitelial motstand jevnlig over 48 h og western blot i både Caco-2 og PBMC.

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