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Microprobe kapillært geleelektroforese massespektrometri for én celle Metabolomics i Live Frog (Xenopus laevis) embryoer
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Microprobe kapillært geleelektroforese massespektrometri for én celle Metabolomics i Live Frog (Xenopus laevis) embryoer

Article DOI: 10.3791/56956-v • 12:16 min • December 22nd, 2017
December 22nd, 2017 •
1, 1, 2, 1,3
1Department of Chemistry, George Washington University, 2Department of Anatomy & Regenerative Biology, George Washington University, 3Department of Chemistry & Biochemistry, University of Maryland, College Park

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Vi beskriver trinn som aktiverer rask i situ utvalg av en liten del av en enkelt celle med høy presisjon og minimal invasjonen bruker kapillær-baserte mikro-sampling, for å lette kjemisk karakterisering av et øyeblikksbilde av metabolsk aktivitet i Live embryo bruker en spesialbygd enkeltcelle kapillært geleelektroforese og massespektrometri plattform.

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Kjemi problemet 130 kapillære geleelektroforese electrospray ionization massespektrometri microsampling metabolomics enkeltcelle Xenopus frosk embryogenesis
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