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Automated Measurement of Cryptococcal Species Polysaccharide Capsule and Cell Body
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Immunology and Infection
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JoVE Journal Immunology and Infection
Automated Measurement of Cryptococcal Species Polysaccharide Capsule and Cell Body

Automated Measurement of Cryptococcal Species Polysaccharide Capsule and Cell Body

DOI:
10.3791/56957-v

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08:08 min

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January 11, 2018

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1Department of Molecular Microbiology and Immunology,Johns Hopkins Bloomberg School of Public Health

Chapters

  • 00:05Title
  • 00:36Preparation of India Ink Slide
  • 01:35Imaging Slide
  • 02:22Use of Algorithm
  • 05:39Results: Automated Measurement of Circular Polysaccharide Capsules of Cryptococcus neoformans
  • 07:22Conclusion

Summary

Automatic Translation

Automatic Translation

This technique describes an automated batch image processor designed to measure polysaccharide capsule and body radii. While initially designed for Cryptococcus neoformans capsule measurements the automated image processor can also be applied to other contrast based detection of circular objects.

Tags

Automated MeasurementCryptococcal SpeciesPolysaccharide CapsuleCell BodyVirulenceIndia Ink SlideH99 StrainBright-field MicroscopeMeasurement AlgorithmQCA.py

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