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流式细胞仪估测原发性急性髓系白血病和患者源性移植中白血病干细胞的诊断和随访
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Cancer Research
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JoVE Journal Cancer Research
Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
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流式细胞仪估测原发性急性髓系白血病和患者源性移植中白血病干细胞的诊断和随访

DOI:
10.3791/56976-v

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09:01 min

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March 26, 2018

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, , , , , , , , ,
1Laboratoire d’hématologie,CHU Lille, 2Univ. Lille, Inserm, CHU Lille, UMR-S 1172 – JPArc – Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer, 3Service d’hématologie pédiatrique, hôpital Jeanne de Flandre,CHU Lille, 4Laboratoire d’hématologie CH Lyon Sud, 5Service des Maladies du Sang, hôpital Claude Huriez,CHU Lille

Chapters

  • 00:04Title
  • 00:58Sample Preparation and Lysis of Red Blood Cells
  • 02:26Patient Derived Xenografts and Staining
  • 04:50Flow Cytometry and Gating Strategy
  • 06:14Results: Parametric Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia
  • 07:57Conclusion

Summary

Automatic Translation

Automatic Translation

通过流式细胞术, 我们勾勒出一种检测白血病干细胞标记物同时表达的协议。我们展示了如何量化三个祖群体和一个假定的多项种群的成熟程度。我们证实了这些人群存在于相应的病人衍生的移植。

Tags

Flow CytometryLeukemia Stem CellsAcute Myeloid LeukemiaPatient-derived XenograftsDiagnosisFollow-upMononuclear CellsDensity GradientRed Blood Cell LysisNSG MouseXenograftSubmandibular Blood Collection

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