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Immunology and Infection
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双光子激光显微镜在细胞膜修复中的应用
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Immunology and Infection
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Immunology and Infection
Cell Membrane Repair Assay Using a Two-photon Laser Microscope
Please note that all translations are automatically generated.
Click here for the English version.
双光子激光显微镜在细胞膜修复中的应用
DOI:
10.3791/56999-v
•
06:35 min
•
January 02, 2018
•
Joshua J. A. Lee
,
Rika Maruyama
,
Hidetoshi Sakurai
,
Toshifumi Yokota
1
Department of Medical Genetics
,
University of Alberta Faculty of Medicine and Dentistry
,
2
Center for iPS Cell Research and Application
,
Kyoto University
Chapters
00:05
Title
00:38
Full-length Dysferlin (DYSF) Plasmid Cell Transfection
03:09
Two-photon Laser Wounding Assay
05:19
Results: Representative Fluorescence Quantification Over Time
06:00
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
双光子激光的细胞膜损伤是一种广泛应用的评价膜加盖能力的方法, 可应用于多种细胞类型。在这里, 我们描述了一个协议的
体外
活成像膜加盖在 dysferlinopathy 患者细胞后双光子激光消融。
Tags
Cell Membrane Repair
Muscular Dystrophy
Two-photon Laser Microscope
Fibroblast Cells
Plasma Membrane
Membrane Resealing
Dysferlin
Transfection
FM4-64 Dye
Two-photon Wounding Assay
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