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来自沙柯玛患者衍生异种移植物的肿瘤启动细胞的分离和表征
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Isolation and Characterization of Tumor-initiating Cells from Sarcoma Patient-derived Xenografts
Please note that all translations are automatically generated.
Click here for the English version.
来自沙柯玛患者衍生异种移植物的肿瘤启动细胞的分离和表征
DOI:
10.3791/57011-v
•
07:18 min
•
June 13, 2019
•
Dan Han
,
Veronica Rodriguez-Bravo
,
Sudeh Izadmehr
,
Josep Domingo-Domenech
,
Carlos Cordon-Cardo
1
Department of Pathology
,
Icahn School of Medicine at Mount Sinai
Chapters
00:04
Title
00:44
Sarcoma Tissue Sample Processing and Patient-derived Xenograft (PDX) Formation
02:32
Tumor-initiating Cell (TIC) Sorting and Sarcosphere Formation
05:11
Results: Representative Human Leukocyte Antigen (HLA)-1-Negative TIC Characterization
06:42
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了通过荧光活性细胞分类,使用人类白细胞抗原-1(HLA-1)作为阴性标记,从人类肉瘤患者衍生的异种移植物分离肿瘤启动细胞的详细方案,以及进一步的验证和这些HLA-1阴性肿瘤启动细胞的表征。
Tags
Tumor-initiating Cells
Sarcoma
Patient-derived Xenografts
HLA-I
Epigenetic Downregulation
Cancer Stem Cells
Immune Escape
Single Cell Suspension
NOD Scid Gamma Mouse
Basement Membrane Matrix
Histological Analysis
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