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基于复用等温放大诊断平台检测 Zika、基孔肯亚和登革热1
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
DOI:

06:18 min

March 13, 2018

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Chapters

  • 00:05Title
  • 00:55RT-LAMP for Detection of Zika-infected Mosquitoes Using Q-paper Technology
  • 02:23Testing Lyophilized RT-LAMP Reagents on Urine Samples Containing Zika
  • 04:05Results: Singleplex and Multiplex RT-LAMP for Detection of Zika, Chikungunya, and Dengue 1
  • 05:40Conclusion

Summary

Automatic Translation

目前用于检测 Zika、基孔肯亚和登革热病毒的复用诊断程序需要复杂的样本准备和昂贵的仪器, 而且在低资源环境下很难使用。我们显示一个诊断, 使用等温放大与目标特定的链不可替代探针, 以检测和区分这些病毒具有高灵敏度和特异性。

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