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Developmental Biology
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老年人刷膜的体外模型对视网膜色素上皮细胞的培养
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Culturing of Retinal Pigment Epithelial Cells on an
Ex Vivo
Model of Aged Human Bruch’s Membrane
Please note that all translations are automatically generated.
Click here for the English version.
老年人刷膜的体外模型对视网膜色素上皮细胞的培养
DOI:
10.3791/57084-v
•
08:32 min
•
April 12, 2018
•
Hui Cai
,
Jie Gong
,
Lucian V. Del Priore
,
Tongalp H. Tezel
,
Mark A. Fields
1
Department of Ophthalmology and Visual Science
,
Yale School of Medicine
,
2
Edward S. Harkness Eye Institute
,
Columbia University School of Medicine
Chapters
00:05
Title
01:04
Harvesting of Human Bruch’s Membrane Explants
04:53
Culturing of Retinal Pigment Epithelial (RPE) Cells on Human Bruch’s Membrane Explants
06:36
Results: Culturing RPE Cells on Aged Bruch’s Membranes
07:19
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本协议的目的是展示视网膜色素上皮 (RPE) 细胞在老年和/或患病的人刷膜的培养。该方法适用于细胞外基质上皮细胞行为的研究。
Tags
Retinal Pigment Epithelial Cells
Bruch’s Membrane
Ex Vivo Model
Aged Human
AMD Etiology
Proteomics
RPE Cell Culture
Sclera
Vitreous
Retina
Optic Nerve
Ammonium Hydroxide
DPBS
PTFE Membrane
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