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高压低温电子层析成像对蓝藻 DNA 压实的可视化研究
JoVE Journal
Biology
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JoVE Journal
Biology
Visualization of DNA Compaction in Cyanobacteria by High-voltage Cryo-electron Tomography
Please note that all translations are automatically generated.
Click here for the English version.
高压低温电子层析成像对蓝藻 DNA 压实的可视化研究
DOI:
10.3791/57197-v
•
09:47 min
•
July 17, 2018
•
Kazuyoshi Murata
,
Yasuko Kaneko
1
National Institute for Physiological Sciences
,
2
Graduate School of Science and Engineering
,
Saitama University
Chapters
00:04
Title
00:43
Synchronous Culture of Cyanobacteria and Monitoring by Fluorescence Microscopy
02:31
Sample Freezing and Cryo-HVET
05:16
Tomographic Reconstruction and Segmentation of the Feature of Interest
07:20
Results: Observations of Compacted DNA in Cyanobacteria
08:36
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了如何可视化蓝藻中的瞬态 DNA 压缩。采用同步培养、荧光显微镜、快速冷冻、高压低温电子层析成像等技术进行监测。提出了这些方法的一个协议, 并讨论了未来的应用和发展。
Tags
DNA Compaction
Cyanobacteria
High-voltage Cryo-electron Tomography
Bacterial Cell Cycle
Ultrastructure
Hoechst Stain
Fluorescence Microscopy
Plunge-freezing
Liquid Nitrogen
Liquid Ethane
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