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Journal / Genetics / CRISPR/Cas9…
JoVE Journal
Genetics

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JoVE Journal Genetics
CRISPR/Cas9 によるマウスおよび人間の造血前駆細胞の高効率遺伝子破壊
Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen
Journal (Genetics)
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An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness
Journal (Genetics)
An Ecdysone Receptor-based Singular Gene Switch for Deliberate Expression of Transgene with Robustness, Reversibility, and Negligible Leakiness
 
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CRISPR/Cas9 によるマウスおよび人間の造血前駆細胞の高効率遺伝子破壊

Article DOI: 10.3791/57278-v • 08:27 min • April 10th, 2018
April 10th, 2018 •
*1,2,3, *1,2,4, 4, 1,2,4, 1,2,4,5
1Stem Cells & Regenerative Medicine Center, Baylor College of Medicine, 2Center for Cell and Gene Therapy, Baylor College of Medicine, 3Centro di Ricerca Emato-Oncologica (CREO), University of Perugia, 4Department of Molecular & Human Genetics, Baylor College of Medicine, 5Texas Children's Hospital & Houston Methodist Hospital
* These authors contributed equally

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高速のためのプロトコル マウスに CRISPR/Cas9 を介した遺伝子破壊とひと造血細胞はこの資料に記載されています。Cas9 sgRNA ribonucleoproteins は、sgRNAsの in vitro転写と商業 Cas9 を介して生成されたとエレクトロポレーションを介して紹介しています。限られた時間と金銭的コスト高の編集効率を実現します。

Tags

遺伝学、問題 134、CRISPR Cas9、HSPCs、造血幹・前駆細胞、ノックアウト、リボ核蛋白質、遺伝子の編集、
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