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JoVE Journal
Genetics

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CRISPR/Cas9 对小鼠和人造血祖细胞的高效基因破坏
 
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CRISPR/Cas9 对小鼠和人造血祖细胞的高效基因破坏

Article DOI: 10.3791/57278
April 10th, 2018

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Summary April 10th, 2018

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Click here for the English version.

本文介绍了一种快速 CRISPR/Cas9-mediated 基因在小鼠和人原发性造血细胞中的破坏的协议。Cas9-sgRNA ribonucleoproteins 是通过电穿孔与 sgRNAs 产生通过体外转录和商业 Cas9。在有限的时间和财务成本下实现了高编辑效率。

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