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利用新的细胞分选策略, 从小鼠骨髓中生成大量的髓样祖细胞和树突状体细胞前体
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Generation of Large Numbers of Myeloid Progenitors and Dendritic Cell Precursors from Murine Bone Marrow Using a Novel Cell Sorting Strategy
DOI:

09:05 min

August 10, 2018

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Chapters

  • 00:04Title
  • 00:40Collection of Murine Bone Marrow Cells
  • 03:57Staining Strategy
  • 05:53Set Gates Based on Control Samples
  • 06:22Collection of Isolated Populations
  • 07:20Results: Robust Myeloid Progenitors Using Protocol
  • 08:43Conclusion

Summary

Automatic Translation

在这里, 我们提供了一种方法, 以识别和隔离大量的 GM-脑脊液驱动髓细胞使用高速细胞排序。根据 Ly6C 和 CD115 的表达, 可以确定五种不同的种群 (常见髓样祖细胞、粒/巨噬细胞祖、单核细胞、单核细胞衍生巨噬细胞和单核细胞衍生的 DCs)。

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