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Developmental Biology
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有丝分裂过程中染色体分离的活体细胞成像
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Live Cell Imaging of Chromosome Segregation During Mitosis
Please note that all translations are automatically generated.
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有丝分裂过程中染色体分离的活体细胞成像
DOI:
10.3791/57389-v
•
06:39 min
•
March 14, 2018
•
Prajakta Varadkar
,
Kazuyo Takeda
,
Brenton McCright
1
Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research
,
US Food and Drug Administration
,
2
Microscopy and Imaging Core facility, Division of Viral Products, Center for Biologics Evaluation and Research
,
US Food and Drug Administration
Chapters
00:04
Title
01:04
Labeling Cells
02:21
Visualization of Lagging Chromosomes
04:45
Results: Nocodazole-treated MEFs Lacking PP2A-B56 Undergo Chromosomal Mis-segregation
05:29
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了一种简便、方便的方法, 用 Histone2B-GFP BacMam 2.0 标签和旋转椎间盘共焦显微系统对有丝分裂细胞中的活染色体进行标记和可视化。
Tags
Live Cell Imaging
Chromosome Segregation
Mitosis
Histone 2B-GFP BacMam 2.0
Spinning Disc Confocal Microscopy
Mouse Embryonic Fibroblasts
Nocodazole
Cell Synchronization
Live Chromosome Visualization
Environmental Chamber
Oil Immersion Objective
Article
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