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Generation af menneskers Nasal epitelcelle Spheroids for individualiseret cystisk fibrose Transmembrane ledningsevne Regulator undersøgelse
JoVE Journal
Medicine
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JoVE Journal Medicine
Generation of Human Nasal Epithelial Cell Spheroids for Individualized Cystic Fibrosis Transmembrane Conductance Regulator Study
DOI:

08:00 min

April 11, 2018

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Chapters

  • 00:04Title
  • 00:41Passage Human Nasal Epithelial Cells
  • 02:45Seeding Cells for HNE Spheroid Cultures
  • 04:37Culture Differentiated and Mature HNE Spheroids
  • 05:27Results: Individualized CFTR Study Through the Generation of HNE Spheroids
  • 07:01Conclusion

Summary

Automatic Translation

Her beskriver vi en metode til at generere tredimensionelle klumpformet kulturer af menneskers nasal epitelceller. Spheroids derefter stimuleres til at drive cystisk fibrose Transmembrane ledningsevne Regulator (CFTR)-afhængige ion og væske sekretion, kvantificere ændringen i klumpformet luminale størrelse som en proxy for CFTR funktion.

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