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Lambda Vælg cII Mutation Detection System
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
The Lambda Select cII Mutation Detection System
DOI:

07:08 min

April 26, 2018

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Chapters

  • 00:04Title
  • 00:52In Vitro Packaging Reaction
  • 01:51Plating the Packaged DNA Samples
  • 03:36Examination of the Titer and the Screening Plates to Determine the Putative cll Mutants, Verification of Mutant Plaques, PCR Amplification, and DNA Sequencing
  • 05:31Results: Use of λ Select cll Assay to Detect Mutations in the cll Transgenes from the Genomic DNA
  • 06:31Conclusion

Summary

Automatic Translation

Vi beskriver en detaljeret protokol for Lambda Vælg cII mutation assay i kulturperler celler af transgene gnavere eller de tilsvarende dyr behandlet med et kemisk/fysisk agent af interesse. Denne tilgang har været meget anvendt for mutagenicitet afprøvning af kræftfremkaldende stoffer i pattedyrsceller.

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