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Chapters
- 00:04Title
- 00:52In Vitro Packaging Reaction
- 01:51Plating the Packaged DNA Samples
- 03:36Examination of the Titer and the Screening Plates to Determine the Putative cll Mutants, Verification of Mutant Plaques, PCR Amplification, and DNA Sequencing
- 05:31Results: Use of λ Select cll Assay to Detect Mutations in the cll Transgenes from the Genomic DNA
- 06:31Conclusion
Vi beskriver en detaljeret protokol for Lambda Vælg cII mutation assay i kulturperler celler af transgene gnavere eller de tilsvarende dyr behandlet med et kemisk/fysisk agent af interesse. Denne tilgang har været meget anvendt for mutagenicitet afprøvning af kræftfremkaldende stoffer i pattedyrsceller.
