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前子宫内胚胎小鼠脑电穿孔和脊髓切片培养迁移 GABAergic 中间神经元的活体成像
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Neuroscience
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JoVE Journal Neuroscience
Ex Utero Electroporation and Organotypic Slice Cultures of Embryonic Mouse Brains for Live-Imaging of Migrating GABAergic Interneurons
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前子宫内胚胎小鼠脑电穿孔和脊髓切片培养迁移 GABAergic 中间神经元的活体成像

DOI:
10.3791/57526-v

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09:50 min

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April 20, 2018

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, , ,
1Centre de recherche du CHU Sainte-Justine, 2Department of Neuroscience,Université de Montréal, 3Department of pathology and cellular biology,Université de Montréal, 4Department of Pediatrics,Université de Montréal

Chapters

  • 00:04Title
  • 01:01Collection of Mouse Embryos
  • 02:51Intraventricular Plasmid Injections and Ex Vivo Electroporation of the MGE
  • 03:53Brain Dissection and Organotypic Slice Cultures
  • 07:16Results: Time-lapse Live-imaging of Electroporated INs in Organotypic Slices Cultured for 48 h.
  • 09:09Conclusion

Summary

Automatic Translation

Automatic Translation

在这里, 我们提供了一个低成本和可靠的方法来产生转脑脊髓切片培养的小鼠胚胎适合共焦显微镜和实时成像技术。

Tags

Ex Utero ElectroporationOrganotypic Slice CulturesEmbryonic Mouse BrainsLive-imagingMigrating GABAergic InterneuronsDevelopmental NeuroscienceGenetic Neurodevelopmental DisordersAutismEpilepsyBiosafety CabinetDissectionCultureNeural Based MediumTransfer PipetteBlack Wax

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