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Ex Utero Elektroporation og Organotypic skive kulturer af embryonale mus hjerner for Live-Imaging af migrerer GABAergic Interneurons
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Neuroscience
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JoVE Journal Neuroscience
Ex Utero Electroporation and Organotypic Slice Cultures of Embryonic Mouse Brains for Live-Imaging of Migrating GABAergic Interneurons

Ex Utero Elektroporation og Organotypic skive kulturer af embryonale mus hjerner for Live-Imaging af migrerer GABAergic Interneurons

DOI:
10.3791/57526-v

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09:50 min

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April 20, 2018

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, , ,
1Centre de recherche du CHU Sainte-Justine, 2Department of Neuroscience,Université de Montréal, 3Department of pathology and cellular biology,Université de Montréal, 4Department of Pediatrics,Université de Montréal

Chapters

  • 00:04Title
  • 01:01Collection of Mouse Embryos
  • 02:51Intraventricular Plasmid Injections and Ex Vivo Electroporation of the MGE
  • 03:53Brain Dissection and Organotypic Slice Cultures
  • 07:16Results: Time-lapse Live-imaging of Electroporated INs in Organotypic Slices Cultured for 48 h.
  • 09:09Conclusion

Summary

Automatic Translation

Automatic Translation

Her, giver vi en billig og pålidelig metode til at generere electroporated hjerne organotypic skive kulturer fra mus embryoner egnet til Konfokal mikroskopi og live imaging teknikker.

Tags

Ex Utero ElectroporationOrganotypic Slice CulturesEmbryonic Mouse BrainsLive-imagingMigrating GABAergic InterneuronsDevelopmental NeuroscienceGenetic Neurodevelopmental DisordersAutismEpilepsyBiosafety CabinetDissectionCultureNeural Based MediumTransfer PipetteBlack Wax

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