Journal
/
/
Ex Utero Elektroporation og Organotypic skive kulturer af embryonale mus hjerner for Live-Imaging af migrerer GABAergic Interneurons
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Ex Utero Electroporation and Organotypic Slice Cultures of Embryonic Mouse Brains for Live-Imaging of Migrating GABAergic Interneurons
DOI:

09:50 min

April 20, 2018

, , ,

Chapters

  • 00:04Title
  • 01:01Collection of Mouse Embryos
  • 02:51Intraventricular Plasmid Injections and Ex Vivo Electroporation of the MGE
  • 03:53Brain Dissection and Organotypic Slice Cultures
  • 07:16Results: Time-lapse Live-imaging of Electroporated INs in Organotypic Slices Cultured for 48 h.
  • 09:09Conclusion

Summary

Automatic Translation

Her, giver vi en billig og pålidelig metode til at generere electroporated hjerne organotypic skive kulturer fra mus embryoner egnet til Konfokal mikroskopi og live imaging teknikker.

Related Videos

Read Article