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Fremspring Force mikroskopi: En metode til at kvantificere kræfter udviklet af celle fremspring
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JoVE Journal Biology
Protrusion Force Microscopy: A Method to Quantify Forces Developed by Cell Protrusions

Fremspring Force mikroskopi: En metode til at kvantificere kræfter udviklet af celle fremspring

DOI:
10.3791/57636-v

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06:37 min

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June 16, 2018

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, , , , , , , ,
1Institut de Pharmacologie et Biologie Structurale,IPBS, Université de Toulouse, CNRS, UPS, 2METi, 3CNRS, LAAS, 4Univ de Toulouse, INSA

Chapters

  • 00:00Title
  • 00:45Preparation of Formvar-coated grids
  • 01:46Measurement of film thickness
  • 02:57Seeding cells on grids
  • 04:00Topography measurements of podosome-induced film deformations
  • 05:13Representative results
  • 06:18Conclusion

Summary

Automatic Translation

Automatic Translation

Her, detalje vi de eksperimentelle teknikker, der anvendes til at evaluere de fremspring kræfter, der podosomes anvendelse på en kompatibel film, fra forberedelse af filmen til den automatiserede analyse af topografiske billeder.

Tags

Protrusion Force MicroscopyAtomic Force MicroscopyCell ProtrusionsFormvar FilmCell AdhesionPodosomesMechanical ModelFilm Thickness MeasurementContact Mode AFM

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