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Biology
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凸出力显微镜: 一种量化细胞突起力的方法
JoVE Journal
Biology
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JoVE Journal
Biology
Protrusion Force Microscopy: A Method to Quantify Forces Developed by Cell Protrusions
Please note that all translations are automatically generated.
Click here for the English version.
凸出力显微镜: 一种量化细胞突起力的方法
DOI:
10.3791/57636-v
•
06:37 min
•
June 16, 2018
•
Anaïs Bouissou*
1
,
Amsha Proag*
1
,
Marion Portes
,
Vanessa Soldan
,
Stéphanie Balor
,
Christophe Thibault
4
,
Christophe Vieu
4
,
Isabelle Maridonneau-Parini
,
Renaud Poincloux
1
Institut de Pharmacologie et Biologie Structurale
,
IPBS, Université de Toulouse, CNRS, UPS
,
2
METi
,
3
CNRS, LAAS
,
4
Univ de Toulouse, INSA
Chapters
00:00
Title
00:45
Preparation of Formvar-coated grids
01:46
Measurement of film thickness
02:57
Seeding cells on grids
04:00
Topography measurements of podosome-induced film deformations
05:13
Representative results
06:18
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们详细介绍了用于评估 podosomes 在兼容薄膜上应用的突出力的实验技术, 从薄膜的制备到地形图像的自动分析。
Tags
Protrusion Force Microscopy
Atomic Force Microscopy
Cell Protrusions
Formvar Film
Cell Adhesion
Podosomes
Mechanical Model
Film Thickness Measurement
Contact Mode AFM
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