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Developmental Biology

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JoVE Journal Developmental Biology
Effektiv Generation af bugspytkirtlen/Duodenum HOXD Protein 1+ Posterior Foregut/pancreas ophav fra hPSCs i vedhæftning kulturer
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Effektiv Generation af bugspytkirtlen/Duodenum HOXD Protein 1+ Posterior Foregut/pancreas ophav fra hPSCs i vedhæftning kulturer

Article DOI: 10.3791/57641-v • 08:32 min • March 27th, 2019
March 27th, 2019 •
1, 1, 1, 1
1Center for iPS Cell Research and Application (CiRA), Kyoto University

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Vi præsenterer her, en detaljeret protokol for at differentiere humane pluripotente stamceller (hPSCs) i bugspytkirtlen/duodenum HOXD protein 1+ (PDX1+) celler for generation af pancreas lineages baseret på ikke-koloni type éncellelag vækst af adskilles enkelt celler. Denne metode er velegnet til producerer homogene hPSC-afledte celler, genmanipulation og screening.

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Udviklingsmæssige biologi sag 145 bugspytkirtlen menneskelige embryonale stamceller menneskelige inducerede pluripotente stamceller humane pluripotente stamceller differentiering udvikling pancreas stamfader posterior foregut regenerativ medicin diabetes
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