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JoVE Journal
Developmental Biology

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高效生成粘附培养中 Hpsc 的胰腺-二叶多布蛋白 1+后后福格内胰腺祖细胞
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高效生成粘附培养中 Hpsc 的胰腺-二叶多布蛋白 1+后后福格内胰腺祖细胞

Article DOI: 10.3791/57641-v
March 27th, 2019


Summary March 27th, 2019

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在这里, 我们提出了一个详细的协议, 以区分人类多能干细胞 (Hpsc) 到胰十二指肠蛋白 1+ (pdx1+) 细胞的产生的非群体类型单层生长的基础上, 胰腺谱系离解的单细胞。该方法适用于同源 hPSC-derived 衍生细胞的产生、遗传操作和筛选。


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