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Immunology and Infection
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三维人体免疫细胞可视化的光片显微术
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Immunology and Infection
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Immunology and Infection
Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells
Please note that all translations are automatically generated.
Click here for the English version.
三维人体免疫细胞可视化的光片显微术
DOI:
10.3791/57651-v
•
09:44 min
•
June 13, 2018
•
Rouven Schoppmeyer
,
Renping Zhao
,
Markus Hoth
,
Bin Qu
1
Department of Biophysics, Center for Integrative Physiology and Molecular Medicine (CIPMM), School of Medicine
,
Saarland University
Chapters
00:04
Title
00:53
Preparation of Neutralized Collagen
01:55
Sample Preparation for Light-sheet Fluorescence Microscopy Using Capillaries
04:08
Image Acquisition Using Light-sheet Microscopy
05:07
Fixation and Immunofluorescence Staining of Cells in Collagen Matrices
07:17
Results: Visualization of Human CTL Cells by Light-sheet Microscopy
08:32
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了一个协议, 以可视化的免疫细胞嵌入在三维 (3D) 胶原基质中使用光片显微镜。该协议还阐述了如何在3D 中跟踪单元迁移。该协议可用于3D 矩阵中其他类型的悬浮单元。
Tags
Light-sheet Microscopy
Three-dimensional Visualization
Human Immune Cells
Immunology
Cell Behavior
Physiologically Relevant Context
Collagen
Fluorescent Labeling
Cell Culture
Capillary
Imaging
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