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JoVE Journal Developmental Biology
Udarbejdelsen af perifere olfaktoriske væv for at udvikle molekylære og immunhistokemisk analyse i Drosophila
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Udarbejdelsen af perifere olfaktoriske væv for at udvikle molekylære og immunhistokemisk analyse i Drosophila

Article DOI: 10.3791/57716-v • 09:18 min • June 13th, 2018
June 13th, 2018 •
1, 1
1Department of Biology, Duke University

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Vi præsenterer her, en protokol for at iscenesætte og dissekere udvikle olfaktoriske væv fra Drosophila arter. De dissekerede væv kan senere bruges til molekylære analyser, som kvantitativ RT-PCR (Reverse transkription-Polymerase Chain Reaction) eller RNA sekventering (RNAseq), samt i vivo analyser som Immunhistokemi eller i situ hybridisering.

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Udviklingsmæssige biologi sag 136 Drosophila arter olfaktoriske systemudvikling antennal disc antenner dissektioner transcriptional profilering
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