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JoVE Journal Developmental Biology
Sporing genuttrykk gjennom påvisning av β-galactosidase aktivitet i hele Mouse embryoer
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Sporing genuttrykk gjennom påvisning av β-galactosidase aktivitet i hele Mouse embryoer

Article DOI: 10.3791/57785-v • 08:42 min • June 26th, 2018
June 26th, 2018 •
*1, *1, 1, 1, 1, 1, 2,3
1Faculty of Biomedical and Health Sciences, Universidad Europea de Madrid, 2Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), 3School of Doctoral Studies and Research, Universidad Europea de Madrid
* These authors contributed equally

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Her beskriver vi standardprotokollen for påvisning av β-galactosidase aktivitet i tidlig hele mouse embryoer og metoden for parafin snitting og counterstaining. Dette er en enkel og rask prosedyre å overvåke genuttrykk under utvikling som kan også brukes til vev deler, organer eller kulturperler celler.

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Utviklingspsykologi biologi problemet 136 LacZ β-galactosidase parafin skjæring musen utvikling embryonale avklaring genuttrykk
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