JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Chemistry
/
局部钙离子梯度对大泡囊膜重塑的响应
JoVE Journal
Chemistry
This content is Free Access.
JoVE Journal
Chemistry
Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients
Please note that all translations are automatically generated.
Click here for the English version.
局部钙离子梯度对大泡囊膜重塑的响应
DOI:
10.3791/57789-v
•
08:15 min
•
July 16, 2018
•
Baharan Ali Doosti
,
Ann-Sofie Cans
,
Gavin D.M. Jeffries
,
Tatsiana Lobovkina
1
Department of Chemistry and Chemical Engineering
,
Chalmers University of Technology
Chapters
00:04
Title
00:41
Preparation of Small Lipid Vesicles
02:38
Preparation of GUV-MLV Complexes
04:12
Micropipette Preparation and Microinjection
06:09
Formation and Translation of Membrane Tubular Protrusions (MTPs) using the Calcium Ion Source
06:57
Results: Formation of Membrane Tubular Protrusions in Giant Vesicles Upon Localized Application of Calcium Ion Gradients
07:37
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
我们提出了一种非接触微操作系统泡的技术, 利用局部钙离子梯度。在巨大的脂质囊的附近注射钙离子溶液, 用于重塑脂质膜, 导致膜管状突起的产生。
Tags
Membrane Remodeling
Giant Vesicles
Calcium Ion Gradients
Lipid Membrane
Cell Membrane Reshaping
GUV-MLV
Small Lipid Vesicles
Lipid Film
Phosphate-buffered Saline
Glycerol
Sonication
Vortex Mixing
Vacuum Desiccation
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
揭开熵率的加速溶剂动力学的膜酶诱导
从构造到晶体 - 迈向β桶外膜蛋白的结构解析
用场不对称离子迁移光谱法分析 2-丙醇在室内空气浓度中的光氧化作用
用于化学诱导的 DNA 化和表观遗传重塑的基因工程 Split-TET2 酶
基质辅助激光解吸/电离质谱中增强碳水化合物离子信号的高效样品制备方法
生物聚合物添加剂中碳酸钙的形成
用于测定金属结合寡聚肽的金属电子识别和机制的Ion流动性-质谱技术
通过电形成和微移液器测量其机械性能,对巨型Unilamellar混合囊泡的吸收
使用二次离子质谱法重建分离杂质的 3D 深度剖面
通过电子压图进行本地化无浴金属复合电镀
Read Article