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Biochemistry
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酵母 2-混合筛在批次中比较蛋白质相互作用
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Biochemistry
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JoVE Journal
Biochemistry
A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions
Please note that all translations are automatically generated.
Click here for the English version.
酵母 2-混合筛在批次中比较蛋白质相互作用
DOI:
10.3791/57801-v
•
14:23 min
•
June 06, 2018
•
Tabitha A. Peterson
,
Mark A. Stamnes
,
Robert C. Piper
1
Molecular Physiology and Biophysics
,
University of Iowa
Chapters
00:04
Title
00:45
Creating Yeast Populations with Bait and Prey Library
08:03
Sample Preparation for DEEPN Deep Sequencing
11:51
Results: DEEPN Screen Allows Wide-scale Protein Interaction Studies
13:00
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
酵母 2-混合筛的分批处理可以直接比较多饵蛋白与高度复杂的猎物融合蛋白的相互作用。在这里, 我们描述了精制方法, 新试剂, 以及如何实现这些屏幕的使用。
Tags
Yeast 2-hybrid
Protein-protein Interactions
Deep Sequencing
Bait Plasmid
Prey Library
MATa
MATα
Centromere
Kanamycin
Gal4 DNA Binding Domain
Immunoblotting
Genomic DNA
Adapter
CSM Medium
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