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鼻电位差量化小鼠跨上皮离子转运
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Biology
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JoVE Journal
Biology
Nasal Potential Difference to Quantify Trans-epithelial Ion Transport in Mice
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鼻电位差量化小鼠跨上皮离子转运
DOI:
10.3791/57934-v
•
08:55 min
•
July 04, 2018
•
Mathilde Beka
,
Teresinha Leal
1
Louvain Center for Toxicology and Applied Pharmacology (LTAP), Institut de Recherche Expérimentale et Clinique (IREC)
,
Université Catholique de Louvain
Chapters
00:04
Title
00:49
Pre-test Assessment and Management
01:59
Assessment and Management During the Test
06:55
Results: Representative Nasal PD Tracings
08:03
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了一个测量小鼠鼻电位差异的协议。该试验量化了囊性纤维化跨膜电导调节剂和上皮钠通道等跨膜离子转运蛋白的功能。评价新疗法对囊性纤维化的疗效有重要意义。
Tags
Nasal Potential Difference
Cystic Fibrosis
Transgenic Mice
Ion Transport
Double Lumen Nasal Catheter
Electrolyte Cream
Voltmeter
Peristaltic Pump
Data Capture Software
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