Journal
/
Biology
/
Nasal potentielle forskel at kvantificere Trans-epitelial Ion Transport i mus
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Nasal Potential Difference to Quantify Trans-epithelial Ion Transport in Mice

Nasal potentielle forskel at kvantificere Trans-epitelial Ion Transport i mus

DOI:
10.3791/57934-v

•

08:55 min

•

July 04, 2018

•

,
1Louvain Center for Toxicology and Applied Pharmacology (LTAP), Institut de Recherche Expérimentale et Clinique (IREC),Université Catholique de Louvain

Chapters

  • 00:04Title
  • 00:49Pre-test Assessment and Management
  • 01:59Assessment and Management During the Test
  • 06:55Results: Representative Nasal PD Tracings
  • 08:03Conclusion

Summary

Automatic Translation

Automatic Translation

Vi præsenterer her, en protokol for at måle nasal spændingsforskel i mus. Testen kvantificerer funktion af transmembrane ion transportvirksomheder som cystisk fibrose transmembrane ledningsevne regulator og epitelial natrium-kanal. Det er værdifuldt at evaluere effekten af nye terapier for cystisk fibrose.

Tags

Nasal Potential DifferenceCystic FibrosisTransgenic MiceIon TransportDouble Lumen Nasal CatheterElectrolyte CreamVoltmeterPeristaltic PumpData Capture Software

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

MALDI Sample Preparation: the Ultra Thin Layer Method

A Cell-to-cell Macromolecular Transport Assay in Planta Utilizing Biolistic Bombardment

Patch Clamp and Perfusion Techniques for Studying Ion Channels Expressed in Xenopus oocytes

Using SCOPE to Identify Potential Regulatory Motifs in Coregulated Genes

Dyrkning af human Nasal epithelialceller på Air Liquid grænseflade

Funktionel karakterisering af Na<sup> +</sup> / H<sup> +</sup> Exchangers af intracellulære Rum Brug Proton-drab Selection at udtrykke dem på plasmamembranen

Anvendelse af enzymatiske Biosensorer at kvantificere Endogen ATP eller H<sub> 2</sub> O<sub> 2</sub> I Nyre

Teknikker til at fremkalde og kvantificere cellesenescens

Modificerede metoder til belastning af høj-throughput DNA ekstraktionsplader reducere risikoen for forurening

Primære humane nasale epitelceller: Biobanking i forbindelse med præcisionsmedicin

Read Article