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Genetics
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用小鼠脾评估病原体相关分子模式对时钟基因表达的影响
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Genetics
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JoVE Journal
Genetics
The Use of Mouse Splenocytes to Assess Pathogen-associated Molecular Pattern Influence on Clock Gene Expression
Please note that all translations are automatically generated.
Click here for the English version.
用小鼠脾评估病原体相关分子模式对时钟基因表达的影响
DOI:
10.3791/58022-v
•
06:50 min
•
July 24, 2018
•
Adam C. Silver
1
Department of Biology
,
University of Hartford
Chapters
00:04
Title
00:31
Media Preparation, Splenocyte Isolation, and Challenge
03:02
RNA Isolation and cDNA Synthesis
04:50
Quantitative Polymerase Chain Reaction (qPCR)
05:40
Results: Clock Gene Expression
06:33
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本协议描述了一种使用小鼠脾的技术来发现改变分子时钟基因表达的病原相关分子模式。
Tags
Mouse Splenocytes
Pathogen-associated Molecular Pattern
Clock Gene Expression
Immunology
Chronobiology
PAMP
Cell Culture
Cell Homogenization
Cell Counting
Cell Stimulation
RNA Extraction
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