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Journal
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Immunology and Infection
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小鼠骨髓 Oligopotent 和世系髓样祖细胞的鉴定与分离
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow
Please note that all translations are automatically generated.
Click here for the English version.
小鼠骨髓 Oligopotent 和世系髓样祖细胞的鉴定与分离
DOI:
10.3791/58061-v
•
07:21 min
•
July 29, 2018
•
Alberto Yáñez
2
,
Helen S. Goodridge
2
1
Board of Governors Regenerative Medicine Institute
,
Cedars-Sinai Medical Center
,
2
Research Division of Immunology
,
Cedars-Sinai Medical Center
Chapters
00:04
Title
00:32
Magnetic-activated Cell Sorting (MACS) for Progenitor Enrichment
02:06
Fluorescence-activated Cell Sorting (FACS) for Myeloid Progenitor Identification and Isolation
06:07
Results: Representative Progenitor Yields and Purities
06:45
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们展示了如何用磁性和荧光分选 (mac 和流动力学) 相结合的方法, 从小鼠骨髓中识别和分离出6个髓样祖细胞。本协议可用于
体外
培养测定 (纤维素或液体培养),
体内
移植实验, RNA/蛋白质分析。
Tags
Myeloid Progenitors
Bone Marrow
MACS
Lineage-negative Cells
FACS
Neutrophils
Monocytes
Dendritic Cells
Cell Isolation
Hematology
Immunology
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