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Udtryk for fluorescerende Fusion proteiner i Murine knoglemarv-afledte dendritiske celler og makrofager
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JoVE Journal Biology
Expression of Fluorescent Fusion Proteins in Murine Bone Marrow-derived Dendritic Cells and Macrophages

Udtryk for fluorescerende Fusion proteiner i Murine knoglemarv-afledte dendritiske celler og makrofager

DOI:
10.3791/58081-v

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09:07 min

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October 30, 2018

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, , ,
1Laboratory of Leukocyte Signalling,Institute of Molecular Genetics of the ASCR, 2Faculty of Science,Charles University, 3Department of Biophysical Chemistry,J. Heyrovsky Institute of Physical Chemistry ASCR

Chapters

  • 00:04Title
  • 00:40Retrovirus Production
  • 02:38Murine Bone Marrow Cell Isolation and Macrophage Differentiation
  • 05:29Bone Marrow-derived Macrophage (BMDM) and Dendritic Cell (BMDC) EGFP-dagged Protein of Interest Production
  • 06:53Results: Representative Fluorescent Fusion Protein Expression in Murine BMDM and BMDC
  • 07:52Conclusion

Summary

Automatic Translation

Automatic Translation

I denne artikel vil give vi en detaljeret protokol for udtryk for fluorescerende fusion proteiner i murine knoglemarv afledt dendritiske celler og makrofager. Metoden er baseret på transduktion af knoglemarv stamceller med retroviral konstruktioner efterfulgt af differentiering af makrofager og dendritiske celler i vitro.

Tags

Fluorescent Fusion ProteinsMurine Bone Marrow-derived Dendritic CellsMacrophagesRetroviral TransductionPlatinum Eco-packaging CellsEcotropic Retroviral ParticlesBone Marrow Harvesting

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