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Udtryk for fluorescerende Fusion proteiner i Murine knoglemarv-afledte dendritiske celler og makrofager
JoVE Journal
Biology
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JoVE Journal Biology
Expression of Fluorescent Fusion Proteins in Murine Bone Marrow-derived Dendritic Cells and Macrophages
DOI:

09:07 min

October 30, 2018

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Chapters

  • 00:04Title
  • 00:40Retrovirus Production
  • 02:38Murine Bone Marrow Cell Isolation and Macrophage Differentiation
  • 05:29Bone Marrow-derived Macrophage (BMDM) and Dendritic Cell (BMDC) EGFP-dagged Protein of Interest Production
  • 06:53Results: Representative Fluorescent Fusion Protein Expression in Murine BMDM and BMDC
  • 07:52Conclusion

Summary

Automatic Translation

I denne artikel vil give vi en detaljeret protokol for udtryk for fluorescerende fusion proteiner i murine knoglemarv afledt dendritiske celler og makrofager. Metoden er baseret på transduktion af knoglemarv stamceller med retroviral konstruktioner efterfulgt af differentiering af makrofager og dendritiske celler i vitro.

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