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Biology
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用 RNA
原位
杂交法对形态学组织环境中的遗传变异、短靶点和突变进行可视化
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Visualizing Genetic Variants, Short Targets, and Point Mutations in the Morphological Tissue Context with an RNA
In Situ
Hybridization Assay
Please note that all translations are automatically generated.
Click here for the English version.
用 RNA
原位
杂交法对形态学组织环境中的遗传变异、短靶点和突变进行可视化
DOI:
10.3791/58097-v
•
10:57 min
•
August 14, 2018
•
Courtney M. Anderson
,
Annelies Laeremans
,
Xiao-Ming Mindy Wang
,
Xingyong Wu
,
Bingqing Zhang
,
Emerald Doolittle
,
Jeffrey Kim
,
Na Li
,
Helly Xiao Yan Pimentel
,
Emily Park
,
Xiao-Jun Ma
1
Advanced Cell Diagnostics, Inc
Chapters
00:04
Title
00:41
Tissue Preparation
01:30
Equipment and Reagent Preparation
03:24
Sample Deparaffinization, Dehydration, and Pre-treatment
05:26
Probe Hybridization, Signal Amplification, and Detection
07:18
Counterstaining, Slide Mounting, and Visualization
08:18
Results: Detection of Splice Variants, Short Sequences, and Point Mutation
In Situ
10:00
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们描述了
原位
杂交检测, 使敏感和特定的检测序列短于50核苷酸的单核苷酸分辨率在单细胞水平。该检测可以手动或自动执行, 可以在组织上下文中实现拼接变体、短序列和突变的可视化。
Tags
Genetic Variants
Short Targets
Point Mutations
RNA In Situ Hybridization
Cell Type Expression
Splice Variants
Neuroscience
Oncology
Exon Junctions
Sensitivity
Specificity
Single Cells
Formalin Fixation
Paraffin Embedding
Microtome Sectioning
Target Retrieval
Wash Buffer
Hematoxylin Counterstain
Probe Hybridization
Amplification Reagents
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