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Isolering og identifikation af ekstravaskulære immunceller af hjertet
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation and Identification of Extravascular Immune Cells of the Heart

Isolering og identifikation af ekstravaskulære immunceller af hjertet

DOI:
10.3791/58114-v

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08:24 min

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August 23, 2018

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, ,
1Toronto General Hospital Research Institute,University Health Network (UHN), 2Dept of Laboratory Medicine and Pathobiology,University of Toronto, 3Dept of Immunology,University of Toronto, 4Peter Munk Cardiac Centre, 5Ted Rogers Centre for Heart Research

Chapters

  • 00:04Title
  • 00:40Circulating Leukocyte Labeling and Analysis
  • 06:22Results: Cardiac Antigen Presenting Cell Quantification
  • 07:53Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol udgør en enkel og effektiv metode til at isolere, identificere og kvantificere immunceller bosat i myokardiet af mus under steady state eller betændelse. Protokollen kombinerer enzymatiske og mekaniske fordøjelsen for generation af en enkelt cellesuspension, der yderligere kan analyseres ved flowcytometri.

Tags

Immune CellsHeartImmunophenotypingCardiac Immune Cell PopulationsTail Vein InjectionFluorophore-conjugated Anti-CD45 AntibodyHeart PerfusionIntravascular Contaminants

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