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倒置方向下整个人的心脏在加压袋内的去细胞化

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Decellularization of Whole Human Heart Inside a Pressurized Pouch in an Inverted Orientation

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倒置方向下整个人的心脏在加压袋内的去细胞化

DOI:

10.3791/58123-v

•

06:28 min

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November 26, 2018

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Doris A. Taylor, Luiz C. Sampaio, Rafael Cabello, Abdelmotagaly Elgalad, Rohan Parikh, R Patrick Wood, Kevin A. Myer, Alvin T. Yeh, Po-Feng Lee

¹Regenerative Medicine Research,Texas Heart Institute, ²Lifegift Organ Donation Center, ³Biomedical Engineering,Texas A&M University

Chapters

00:04Title
00:38Organ Preparation and Decellularization
03:38Results: Representative Whole Human Heart Decellularization Flow Rate Analysis
05:18Conclusion

Summary

Automatic Translation

这种方法可以使用基于渗透冲击的简单协议和最小器官破坏矩阵的离子洗涤剂灌注, 使复杂的固体器官去极化。它包括一种新的人的心脏在加压袋内的去电磁化技术, 并实时监测流动动力学和细胞碎片流出。

Tags

Decellularization Whole Human Heart Pressurized Pouch Inverted Orientation Vascularized Scaffold Septal Defect Vena Cava Aorta Pulmonary Artery Pulmonary Vein Perfusion Polyester Pouch PBS Hypertonic Solution Hypotonic Solution Sodium Dodecyl Sulfate SDS Peracetic Acid

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