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Biology
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人間のサブタイプに固有の光の活動電位記録誘導多能性幹細胞由来の心室心筋細胞
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JoVE Journal
Biology
Subtype-specific Optical Action Potential Recordings in Human Induced Pluripotent Stem Cell-derived Ventricular Cardiomyocytes
Please note that all translations are automatically generated.
Click here for the English version.
人間のサブタイプに固有の光の活動電位記録誘導多能性幹細胞由来の心室心筋細胞
DOI:
10.3791/58134-v
•
06:59 min
•
September 27, 2018
•
Alexander Goedel*
1,2
,
Dorota M. Zawada*
1
,
Fangfang Zhang
,
Zhifen Chen
,
Alessandra Moretti
2
,
Daniel Sinnecker
2
1
Medical Department I, University Hospital Klinikum rechts der Isar
,
Technical University of Munich
,
2
German Centre for Cardiovascular Research (DZHK), Munich Heart Alliance
,
3
Beth Israel Deaconess Medical Center
,
Harvard Medical School
Chapters
00:04
Title
00:50
Induced Pluripotent Stem Cell (iPS Cell)-derived Cardiomyocyte Preparation and Optical Membrane Potential Recording
03:25
Analysis
04:55
Results: Representative Optical Action Potential Measurement Analyses
06:36
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
ここで具体的には心室のような誘導多能性幹細胞由来心筋細胞イメージ活動電位光学的方法を提案する.メソッドは、電位感受性蛍光タンパク質のプロモーターに駆動される式に基づいています。
Tags
Human Induced Pluripotent Stem Cell-derived Ventricular Cardiomyocytes
Optical Action Potential Recordings
Genetically Encoded Voltage Sensors
Lentiviral Transduction
Cardiomyocyte Maintenance Medium
Tyrode’s Solution
Epifluorescence Microscope
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