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Journal
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Biochemistry
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体外
支持性脂质双层自组织蛋白模式的重组
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
In Vitro
Reconstitution of Self-Organizing Protein Patterns on Supported Lipid Bilayers
Please note that all translations are automatically generated.
Click here for the English version.
体外
支持性脂质双层自组织蛋白模式的重组
DOI:
10.3791/58139-v
•
08:10 min
•
July 28, 2018
•
Beatrice Ramm*
1
,
Philipp Glock*
1
,
Petra Schwille
1
Department of Cellular and Molecular Biophysics
,
Max Planck Institute of Biochemistry
Chapters
00:04
Title
00:24
Small Unilamellar Vesicle (SUV) Preparation
01:20
Cleaning Glass Coverslips
02:13
Chamber Assembly and Supported Lipid Bilayer (SLB) Formation
04:15
PDMS microstructures
05:48
Self-organization in PDMS Microstructures
07:11
Results: MinDE Self-Organization on Supported Lipid Bilayers
07:44
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们提供了一个协议,
在体外
自我组织的精神和矿山的研究, 在支持的脂质双层在开放室。此外, 我们还描述了如何将该检测包裹在脂质包覆的 microcompartments 中, 通过反应约束模拟
体内
条件。
Tags
In Vitro Reconstitution
Self-organizing Protein Patterns
Supported Lipid Bilayers
Geometric Cues
Pattern Formation
Multilamellar Vesicles
Freeze-thaw
Lipid Extruder
Sulfuric Acid
Hydrogen Peroxide
Coverslips
Supported Lipid Bilayer Formation
Heat Block
Article
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