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以此 T7 噬菌体的定量 PCR 研究
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Immunology and Infection
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JoVE Journal Immunology and Infection
Quantitative PCR of T7 Bacteriophage from Biopanning
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以此 T7 噬菌体的定量 PCR 研究

DOI:
10.3791/58165-v

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05:42 min

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September 27, 2018

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, , , ,
1Division of Molecular Pharmaceutics and Drug Delivery, College of Pharmacy,University of Texas at Austin

Chapters

  • 00:04Title
  • 00:43Prepare Standards for Quantification of T7 Phage Genomic DNA
  • 01:30Pre-treatment of Phage Samples Before qPCR Reaction Preparation
  • 02:29Prepare qPCR Reactions and qPCR Cycling Conditions
  • 03:31Results: Quantitative PCR of T7 Bacteriophage
  • 04:49Conclusion

Summary

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本文介绍了一种可重现的、准确的、时间有效的定量 PCR (qPCR) 方法来枚举 T7 噬菌体。该协议明确描述了噬菌体基因组 DNA 制备、PCR 反应制备、qPCR 循环条件和 qPCR 数据分析。

Tags

Quantitative PCRT7 BacteriophageBiopanningVirologyMicrobiologyPhage EnumerationPhage DiagnosticsPhage TherapeuticsPhage DisplayPrimer DesignReference DNASerial DilutionGenome CopiesDNase TreatmentHeat InactivationQPCR Premix96-well PlateFluorescencePhotobleaching

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